Regulatory

Part:BBa_K4399000:Design

Designed by: Ruyi Shi   Group: iGEM22_Worldshaper-Shanghai   (2022-09-20)


PAtUBI5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1342
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1342
    Illegal NheI site found at 1244
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1342
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1342
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1342
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We employed PAtUBI5 to drive expression of GFP, as a reporter, which confers constitutive expression of GFP. So successful transformants can be screened using GFP fluorescence.


Source

From Arabidopsis (Arabidopsis thaliana) genomic sequence.


References